报告题目：Human Mesenchymal Stem Cell for Stroke Treatment: 3D Aggregation-mediated Functional Activation and High Field MRI Imaging

报 告 人：Teng Ma （The Florida State University USA）

报告时间：2015年7月08日下午14：30

报告地点：化学楼二楼一号会议室

报告人简介：

Prof. Ma has been working in the field of human mesenchymal stem or stromal cell (hMSC) expansion and tissue regeneration for over 10 years. As a bioengineer, He is motivated in developing the enabling technologies that bridge the gap between laboratory findings and their clinical translation. He is committed to the technology development for innovative hMSC expansion and bioprocessing strategies for several reasons. They believe the implementation of the knowledge in hMSC bioprocessing and production is a crucial step in hMSC’s translation to therapeutic application.

报告简介：

         Ischemic stroke is a cerebral infraction that leads to many serious brain damage. Human mesenchymal stem cells (hMSCs) have shown profound therapeutic benefit in stroke lesion recovery by trophic factor secretion or immune-modulation. However, studies have indicated that prolonged adherent culture led to a loss of primitive properties, resulting decreased homing, survival, and secretory function, thereby affect their therapeutic outcome. Culturing cells in three-dimension has been suggested to more closely mimic their in vivo microenvironment than traditional two-dimensional cultures, and yield enhanced multiple cell properties. In this study, the therapeutic properties of 3D aggregate cultured hMSCs were evaluated with regard to stoke therapy. Superparamagnetic iron oxide (SPIO) nanoparticles labeled MSCs, which were derived from 3D aggregation culture, were transplanted into Sprague-Dawley rats middle cerebral artery occlusion (MCAO). In vivo lifespan and stroke lesion recovery wereassessed by performing serial MRI at 21.1 T. to acquire 1H and 23Na images of lesion progression and cell migration.  This study shows that hMSCs cultured as cell aggregates have enhanced lesion recovery compared to adherent cells as measured with 1H and 23Na sequences. The 21.1-T magnet provides increased 23Na sensitivity for measurements of TSC, with potential for identifying penumbral regions.The enhanced recovery of stroke lesion by hMSCs dissociated from cell aggregates is possibly due to their enhanced survival ability, migration ability, and cytokine secretory function. The production of 3D hMSC aggregates in bioreactor is also discussed.